Abstract Details

Presented By:Dharia, Sameera
Affiliated with:University of Utah, Bioengineering
Authors:Dharia, S., Dittami, G., Rabbitt, R.
From:University of Utah
Title
Single-Cell Electric Impedance Topography
Abstract

Single-Cell Electric Impedance Topography (sceTopo) is a non-invasive method under development to measure passive and excitable dielectric/conductance properties of cell membranes with millisecond temporal resolution and subcellular spatial resolution. Here we report results using a new sceTopo platform where cells were positioned in the center of a planar, circumferentially distributed, electrode array. Radio waves (RF, 10 kHz-1 MHz) were passed between extracellular electrodes, and the cell was simultaneously excited by whole-cell voltage clamp. Changes in membrane admittance in response to cellular depolarization were measured using the sceTopo platform. Xenopous Oocytes were selected as a cellular model for development of the sceTopo system due to their large size and suitability for exogenous membrane-spanning protein expression. Preliminary results collected using oocytes expressing Shaker (potassium ion channel) variants indicate that sceTopo may have the potential to monitor membrane-bound protein mobility during cellular excitation.

Supported by: NIH NIDCD R01-DC004928, NSF IGERT DGE-9987616, R44 HL076906-03, R43 MH079695-01