Abstract Details

Presented By:Gooch, Nathan
Affiliated with:University of Utah, Department of Bioengineering
Authors:Nathan Gooch, Vladimir Hlady
From:University of Utah
Title
The influence of poly(ethylene oxide) surface density gradients on human serum albumin adsorption
Abstract

Adsorption of plasma proteins onto biomaterials in contact with blood often results in platelet adhesion and activation, leading to thrombosis and compromising the implanted device. Because platelet adhesion is related to protein adsorption, it is desirable to inhibit the adsorption of blood proteins to blood-contacting materials.

Surface-grafted poly(ethylene oxide) (PEO) has been shown to effectively reduce protein adsorption to surfaces. In order to better understand and quantify the effect of grafted PEO chains, methoxypolyethylene oxide maleimide chains (Mw = 4714 Da) were grafted to silanized fused silica surfaces containing the surface gradient of reactive thiol groups. These surface thiol gradients were created by progressive UV oxidation of the uniform thiol-modified silica surfaces. The PEO surface gradients were characterized using water contact angle measurements and x-ray photoelectron spectroscopy (XPS) and used to study human serum albumin (HSA) adsorption and desorption kinetics. HSA adsorption was measured using total internal reflection fluorescence microscopy (TIRF) technique. The gradient surfaces showed decreasing HSA adsorption as PEO density increased. Also, HSA desorbed more readily as PEO density increased.