Biomedical Polymers Laboratory

Bioanalytical sensing and microarrays, cell-surface interactions, infection and bacterial-surface interactions, drug delivery, antibody drugs, novel vaccines

Research Areas:
• Polymeric biomaterials interactions with cells
• Medical device infection and bacterial colonization
• Nucleic acid and protein microarray performance
• Macrophage interactions with biomaterials
• Novel live vaccine and protein delivery
• Bioanalytical sensing and device miniaturization

New Technical Book Chapters:

• C-Y Lee, L.J. Gamble, G.M. Harbers, P. Gong, D.W. Grainger, D.G. Castner, “Multi-technique characterization of DNA-modified surfaces for biosensing and diagnostic applications” in “Surface Analysis and Techniques in Biology”, ed. V. S. Smentkowski, Springer, New York, NY, in press, 2008.

• M.L. Godek, D.W. Grainger, “The Macrophage in Wound Healing Surrounding Implanted Devices”, in Implantable Glucose Sensing, Wiley, NY, in press, 2008.

• B. von Rechenberg, L. Meinel, D.W. Grainger, M. Hofman-Amtenbrink, “Introduction to Biotechnology”, in AO Principles of Fracture Management, (2nd ed.), T. Ruedi, ed., AO Publishing, AO Foundation, Davos, Switzerland, pp.47-68, 2007.

P. Gong, D.W. Grainger, “Non-Fouling Surfaces: A Review of Principles and Applications for Microarray Capture Assay Designs” in Microarrays: Methods and Protocols, 2nd Ed. (Methods in Molecular Biology), R. Rampal, ed., Humana Press, NJ, USA, pp. 59-92, 2007.

• D.W. Grainger, C.A. Greef, P. Gong, M. Lochhead, “Surface Chemistry in Commercial Microarraying” in Microarrays: Methods and Protocols 2nd ed., (Methods in Molecular Biology), R. Rampal, ed., Humana Press, NJ, USA, pp. 37-57, 2007.


Recent research publications not cited in PubMed databases:

• N. Ayres, D. J. Holt, C.F. Jones, L. E. Corum and D. W. Grainger*Polymer Brushes Containing Sulfonated Sugar
Repeat Units: Synthesis, Characterization and In Vitro Testing of Blood Coagulation Activation, J. Polym. Sci, A, Polym Chem 46: 7713-7724, 2008.
• H. Takahashi, M. Dubey, K. Emoto ,DG. Castner, DW Grainger, “Imaging surface immobilization chemistry: correlation with cell patterning on non-adhesive hydrogel thin films”, Adv. Funct. Mater., 18 1-10 (2008);
• D.W. Grainger, D.G. Castner, “Nanobiomaterials and nanoanalysis: how to improve the nanoscience for biotechnology”, Adv. Mater. 20, 867–877 (2008).
• C. M. Santoro, N.L. Duchsherer, D. W. Grainger, “Minimal antimicrobial efficacy and ocular cell toxicity from silver nanoparticles”, Nanobiotechnology, 10.1007/s12030-008-9007-z (published on line June 1, 2007); 3:2, 55-65 (2007).
• J.A. Auer, A. Goodship , S. Arnoczky, S. Pearce, J. Price, L. Claes , B. von Rechenberg, M. Hofmann-Amtenbrinck, E. Schneider, R. Muller-Terpitz, F. Thiele, K-P. Rippe, D. W. Grainger, “Refining animal models in fracture research: seeking consensus for optimization of both welfare and scientific validity for appropriate biomedical use”, BMC Musculoskel. Disorders 8:72-78 (2007), doi:10.1186/1471-2474-8-72, published on-line August, 2007.
• G. Harbers, K. Emoto, C. Greef. S. Metzger, D.W. Grainger, M. Lochhead, “A functionalized poly(ethylene glycol) bioassay surface chemistry facilitates bio-immobilization and inhibits non-specific protein, bacterial, and mammalian cell adhesion,” Chem. Mater., 19, 4405-4414 (2007).

Park K, Bae YH, Grainger DW, The 14th International Symposium on Recent Advances in Drug Delivery Systems, February 15-18, 2009, Salt Lake City, UT, USA. J Control Release 2009 Sep 12;():

Jones CF, Grainger DW, In vitro assessments of nanomaterial toxicity. Adv Drug Deliv Rev 2009 Jun 21;61(6):438-56

Grainger DW, Nanotoxicity assessment: all small talk? Adv Drug Deliv Rev 2009 Jun 21;61(6):419-21

Ayres N, Holt DJ, Jones CF, Corum LE, Grainger DW, Polymer Brushes Containing Sulfonated Sugar Repeat Units: Synthesis, Characterization and In Vitro Testing of Blood Coagulation Activation. J Polym Sci A Polym Chem 2008;46(23):7713-7724

Grainger DW, Peer review as professional responsibility: a quality control system only as good as the participants. Biomaterials 2007 Dec;28(34):5199-203

Takahashi H, Letourneur D, Grainger DW, Delivery of large biopharmaceuticals from cardiovascular stents: a review. Biomacromolecules 2007 Nov;8(11):3281-93

Cheng F, Gamble LJ, Grainger DW, Castner DG, X-ray Photoelectron Spectroscopy, Time-of-Flight Secondary Ion Mass Spectrometry, and Principal Component Analysis of the Hydrolysis, Regeneration, and Reactivity of N-Hydroxysuccinimide-Containing Organic Thin Films. Anal Chem 2007 Nov 15;79(22):8781-8788

Saldarriaga Fernandez IC, van der Mei HC, Lochhead MJ, Grainger DW, Busscher H , The inhibition of the adhesion of clinically isolated bacterial strains on multi-component cross-linked poly(ethylene glycol)-based polymer coatings. Biomaterials 2007 Oct;28(28):4105-12

Lee CY, Harbers GM, Grainger DW, Gamble LJ, Castner D , Fluorescence, XPS, and TOF-SIMS Surface Chemical State Image Analysis of DNA Microarrays. J Am Chem Soc 2007 Jul 11;():

Lee CY, Nguyen PC, Grainger DW, Gamble LJ, Castner D , Structure and DNA Hybridization Properties of Mixed Nucleic Acid/Maleimide-Ethylene Glycol Monolayers. Anal Chem 2007 Jun 15;79(12):4390-4400

Kuijer R, Jansen EJ, Emans PJ, Bulstra SK, Riesle J, Pieper J, Grainger DW, Busscher H , Assessing infection risk in implanted tissue-engineered devices. Biomaterials 2007 Jun 25;():

Dandy DS, Wu P, Grainger D , Array feature size influences nucleic acid surface capture in DNA microarrays. Proc Natl Acad Sci U S A 2007 May 15;104(20):8223-8

Xue D, Elliott CM, Gong P, Grainger DW, Bignozzi CA, Caramori , Indirect electrochemical sensing of DNA hybridization based on the catalytic oxidation of cobalt (II). J Am Chem Soc 2007 Feb 21;129(7):1854-5

Grainger DW, Greef CH, Gong P, Lochhead MJ, Current microarray surface chemistries. Methods Mol Biol 2007;381():37-57

Godek ML, Sampson JA, Duchsherer NL, McElwee Q, Grainger D , Rho GTPase protein expression and activation in murine monocytes/macrophages is not modulated by model biomaterial surfaces in serum-containing in vitro cultures. J Biomater Sci Polym Ed 2006 Oct;17(10):1141-1158

Godek ML, Malkov GS, Fisher ER, Grainger D , Macrophage Serum-Based Adhesion to Plasma-Processed Surface Chemistry is Distinct from That Exhibited by Fibroblasts. Plasma Process Polym 2006 Aug 15;3(6-7):485-497

Lee CY, Gong P, Harbers GM, Grainger DW, Castner DG, Gamble L , Surface coverage and structure of mixed DNA/Alkylthiol monolayers on gold: characterization by XPS, NEXAFS, and fluorescence intensity measurements. Anal Chem 2006 May 15;78(10):3316-25

Gong P, Harbers GM, Grainger D , Multi-technique comparison of immobilized and hybridized oligonucleotide surface density on commercial amine-reactive microarray slides. Anal Chem 2006 Apr 1;78(7):2342-51

Wu P, Grainger D , Drug/device combinations for local drug therapies and infection prophylaxis. Biomaterials 2006 Apr;27(11):2450-67

Christie RJ, Findley DJ, Dunfee M, Hansen RD, Olsen SC, Grainger D , Photopolymerized hydrogel carriers for live vaccine ballistic delivery. Vaccine 2006 Feb 27;24(9):1462-9

Wu P, Hogrebe P, Grainger D , DNA and protein microarray printing on silicon nitride waveguide surfaces. Biosens Bioelectron 2006 Jan 15;21(7):1252-63

Grainger D , Controlled-release and local delivery of therapeutic antibodies. Expert Opin Biol Ther 2004 Jul;4(7):1029-44