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Vladimir  Hlady
E-mail:

Phone: 801-581-5042
Phone: 801-581-5188 (lab)
Office: 108A BPRB

Vladimir Hlady

Professor of Biomedical Engineering
Adjunct Professor, Departments of Medicinal Chemistry and Materials Science and Engineering
Professor
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AIMBE College of Fellows
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BS Chemistry, 1972, University of Zagreb
MS Chemistry, 1975, University of Zagreb
DSc Chemistry, 1981, University of Zagreb



Research

Research in the Proteins - Polymers - Interfaces Group (PPIG) focuses on proteins and other macromolecules at biomaterial-host tissue interfaces.


Current Research

Recent research themes include:
- evaluating the role of upstream agonists on downstream platelets-biomaterial surface interactions,
- developing microfluidic assays for measuring the efficacy of anti-platelet agents,
- studying the role of astrocyte-expressed glycosaminoglycans in neuronal regeneration,
- measuring micromechanical properties of endothelial glycocalyx in lung microvasculature, and
- evaluating the role of biomaterial surface chemistry on the proteins-surface interactions and dynamics.

Our main approach is to observe interfacial events involving few molecules at short length and time scales. Surface Analysis and Imaging Laboratory utilizes various experimental techniques such as FCS, TIRF, SFM, QCM, SPR and others.

Most recent publications from Proteins - Polymers - Interfaces Group (PPIG) are:
• S.M. Rahman, C.D. Eichinger and V. Hlady, Effects of Upstream Shear Forces on Priming Downstream Adhesion and Activation, Acta Biomaterialia 73 (2018) 228–235; doi: 10.1016/j.actbio.2018.04.002
• J Saikia, R Mohammadpour, M Yazdimamaghani, H Northrup, V Hlady and H. Ghandehari, Silica Nanoparticle–Endothelial Interaction: Uptake and Effect on Platelet Adhesion under Flow Conditions, ACS Applied Bio Materials 1 (5), (2018) 1620-1627, doi: 10.1021/acsabm.8b00466


Selected Publications

Hsiao TW, Tresco PA, Hlady V, Astrocyte spreading and migration on aggrecan-laminin dot gradients. Biointerphases 2017 Sep 11;13(1):01A401

Eichinger CD, Hlady V, Binary agonist surface patterns prime platelets for downstream adhesion in flowing whole blood. Biointerphases 2017 Apr 28;12(2):02C406

Job KM, O'Callaghan R, Hlady V, Barabanova A, Dull RO, The Biomechanical Effects of Resuscitation Colloids on the Compromised Lung Endothelial Glycocalyx. Anesth Analg 2016 Aug;123(2):382-93

Eichinger CD, Fogelson AL, Hlady V, Functional assay of antiplatelet drugs based on margination of platelets in flowing blood. Biointerphases 2016 Jun 30;11(2):029805

Gooch NW, Hlady V, Two surface gradients of polyethylene glycol for a reduction in protein adsorption. Surf Innov 2015 Sep;3(3):172-180

Hsiao TW, Tresco PA, Hlady V, Astrocytes alignment and reactivity on collagen hydrogels patterned with ECM proteins. Biomaterials 2015 Jan;39:124-30

Hsiao TW, Swarup VP, Eichinger CD, Hlady V, Cell substrate patterning with glycosaminoglycans to study their biological roles in the central nervous system. Methods Mol Biol 2015;1229:457-67

Swarup VP, Hsiao TW, Zhang J, Prestwich GD, Kuberan B, Hlady V, Exploiting differential surface display of chondroitin sulfate variants for directing neuronal outgrowth. J Am Chem Soc 2013 Sep 11;135(36):13488-94

Hsiao TW, Swarup VP, Kuberan B, Tresco PA, Hlady V, Astrocytes specifically remove surface-adsorbed fibrinogen and locally express chondroitin sulfate proteoglycans. Acta Biomater 2013 Jul;9(7):7200-8

Swarup VP, Mencio CP, Hlady V, Kuberan B, Sugar glues for broken neurons. Biomol Concepts 2013 Jun;4(3):233-57

Job KM, Dull RO, Hlady V, Use of reflectance interference contrast microscopy to characterize the endothelial glycocalyx stiffness. Am J Physiol Lung Cell Mol Physiol 2012 Jun 15;302(12):L1242-9

Hodgkinson GN, Tresco PA, Hlady V, The role of well-defined patterned substrata on the regeneration of DRG neuron pathfinding and integrin expression dynamics using chondroitin sulfate proteoglycans. Biomaterials 2012 Jun;33(17):4288-97

Meng F, Hlady V, Tresco PA, Inducing alignment in astrocyte tissue constructs by surface ligands patterned on biomaterials. Biomaterials 2012 Feb;33(5):1323-35

Corum LE, Hlady V, The effect of upstream platelet-fibrinogen interactions on downstream adhesion and activation. Biomaterials 2012 Feb;33(5):1255-60

Eichinger CD, Hsiao TW, Hlady V, Multiprotein microcontact printing with micrometer resolution. Langmuir 2012 Jan 31;28(4):2238-43

Ding YX, Hlady V, Competitive Adsorption of Three Human Plasma Proteins onto Sulfhydryl-to-sulfonate Gradient Surfaces. Croat Chem Acta 2011 Oct;84(2):193-202

O'Callaghan R, Job KM, Dull RO, Hlady V, Stiffness and heterogeneity of the pulmonary endothelial glycocalyx measured by atomic force microscopy. Am J Physiol Lung Cell Mol Physiol 2011 Sep;301(3):L353-60

Corum LE, Eichinger CD, Hsiao TW, Hlady V, Using microcontact printing of fibrinogen to control surface-induced platelet adhesion and activation. Langmuir 2011 Jul 5;27(13):8316-22

Stewart RJ, Ransom TC, Hlady V, Natural Underwater Adhesives. J Polym Sci B Polym Phys 2011 Jun;49(11):757-771

Deshmukh V, Britt DW, Hlady V, Excess fibrinogen adsorption to monolayers of mixed lipids. Colloids Surf B Biointerfaces 2010 Dec 1;81(2):607-13

Ding YX, Streitmatter S, Wright BE, Hlady V, Spatial variation of the charge and sulfur oxidation state in a surface gradient affects plasma protein adsorption. Langmuir 2010 Jul 20;26(14):12140-6

Corum LE, Hlady V, Screening platelet-surface interactions using negative surface charge gradients. Biomaterials 2010 Apr;31(12):3148-55

Miller C, Komunjer L, Hlady V, Heterogeneous Nucleation of Dicalcium Phosphate Dihydrate on Modified Silica Surfaces. Med Vjesn (Osijek) 2010;42(3-4):63-72